Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies.

@article{Gupta2016DynamicNP,
  title={Dynamic Nuclear Polarization Enhanced MAS NMR Spectroscopy for Structural Analysis of HIV-1 Protein Assemblies.},
  author={Rupal Gupta and Manman Lu and Guangjin Hou and Marc Anthony Caporini and Melanie Madeleine Rosay and Werner E. Maas and Jochem Struppe and Christopher L. Suiter and Jinwoo Ahn and In-Ja L Byeon and W Trent Franks and Marcella Orwick-Rydmark and Andrea Bertarello and Hartmut Oschkinat and Anne Lesage and Guido Pintacuda and Angela M. Gronenborn and Tatyana Polenova},
  journal={The journal of physical chemistry. B},
  year={2016},
  volume={120 2},
  pages={
          329-39
        }
}
Mature infectious HIV-1 virions contain conical capsids composed of CA protein, generated by the proteolytic cleavage cascade of the Gag polyprotein, termed maturation. The mechanism of capsid core formation through the maturation process remains poorly understood. We present DNP-enhanced MAS NMR studies of tubular assemblies of CA and Gag CA-SP1 maturation intermediate and report 20-64-fold sensitivity enhancements due to DNP at 14.1 T. These sensitivity enhancements enabled direct observation… CONTINUE READING
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