Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification.

@article{Qiu2015DynamicMO,
  title={Dynamic Monitoring of MicroRNA-DNA Hybridization Using DNAase-Triggered Signal Amplification.},
  author={Xiaopei Qiu and Xing Liu and Wei Zhang and Hong Zhang and Tianlun Jiang and Dongli Fan and Yang Luo},
  journal={Analytical chemistry},
  year={2015},
  volume={87 12},
  pages={6303-10}
}
Dynamically monitoring microRNA (miRNA)-DNA reactions is critical for elucidating various biological processes. However, traditional strategies fail to capture this dynamic event because the original targets are preamplified. In the present study, we developed an amplification-free strategy for real-time monitoring of miRNA-DNA hybridization that integrates the advantages of both duplex-specific nuclease (DSN)-triggered signal amplification and single-stranded DNA probe coating facilitated by… CONTINUE READING