Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine.

Abstract

We applied dynamic combinatorial chemistry (DCC) to identify ligands of ThiT, the S-component of the energy-coupling factor (ECF) transporter for thiamine in Lactococcus lactis. We used a pre-equilibrated dynamic combinatorial library (DCL) and saturation-transfer difference (STD) NMR spectroscopy to identify ligands of ThiT. This is the first report in which DCC is used for fragment growing to an ill-defined pocket, and one of the first reports for its application with an integral membrane protein as target.

DOI: 10.1002/cmdc.201700440

Cite this paper

@article{Monjas2017DynamicCC, title={Dynamic Combinatorial Chemistry to Identify Binders of ThiT, an S-Component of the Energy-Coupling Factor Transporter for Thiamine.}, author={Leticia Monjas and Lotteke J Y M Swier and Inda Setyawati and Dirk-Jan Slotboom and Anna K H Hirsch}, journal={ChemMedChem}, year={2017}, volume={12 20}, pages={1693-1696} }