Dynamic Ca2+-dependent stimulation of vesicle fusion by membrane-anchored synaptotagmin 1.


In neurons, synaptotagmin 1 (Syt1) is thought to mediate the fusion of synaptic vesicles with the plasma membrane when presynaptic Ca2+ levels rise. However, in vitro reconstitution experiments have failed to recapitulate key characteristics of Ca2+-triggered membrane fusion. Using an in vitro single-vesicle fusion assay, we found that membrane-anchored… (More)
DOI: 10.1126/science.1187722


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