Dynamic ATP Recycling for Continuous NADP Production

  title={Dynamic ATP Recycling for Continuous NADP Production},
  author={Osato Miyawaki and Kozo Nakamura and Toshimasa Yano},
The conjugated enzyme system of NAD kinase (NK) and acetate kinase (AK) was investigated to produce NADP continuously by the dynamic recycling of ATP. The enzymes were physically entrapped in an ultrafiltration hollow fiber tube and the cosubstrate, ATP, as well as the substrates were fed to the reactor continuously. The ATP turnover increased with a decrease in the concentration of ATP fed and with an increase in the concentration of immobilized enzymes. The maximal productivity of the reactor… CONTINUE READING