Dwelling in the dark: procedures for the crystallography of phytochromes and other photochromic proteins.

Abstract

Crystallization of phytochromes and other photochromic proteins is hampered by the conformational changes that they undergo on exposure to light. As a canonical phytochrome, cyanobacterial Cph1 switches between two stable states upon absorption of red/far-red light. Consequently, it is mandatory to work in darkness from protein purification to crystal… (More)
DOI: 10.1107/S0907444909034106

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