Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells

@article{Elbashir2001DuplexesO2,
  title={Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells},
  author={Sayda M. Elbashir and Jens Harborth and Winfried Lendeckel and Abdullah Yalçin and Klaus Weber and Thomas Tuschl},
  journal={Nature},
  year={2001},
  volume={411},
  pages={494-498}
}
RNA interference (RNAi) is the process of sequence-specific, post-transcriptional gene silencing in animals and plants, initiated by double-stranded RNA (dsRNA) that is homologous in sequence to the silenced gene. The mediators of sequence-specific messenger RNA degradation are 21- and 22-nucleotide small interfering RNAs (siRNAs) generated by ribonuclease III cleavage from longer dsRNAs. Here we show that 21-nucleotide siRNA duplexes specifically suppress expression of endogenous and… Expand

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