Duplex shuttle PCR for differential diagnosis of budgerigar fledgling disease and psittacine beak and feather disease.

@article{Ogawa2005DuplexSP,
  title={Duplex shuttle PCR for differential diagnosis of budgerigar fledgling disease and psittacine beak and feather disease.},
  author={Hirohito Ogawa and Tsuyoshi Yamaguchi and Hideto Fukushi},
  journal={Microbiology and immunology},
  year={2005},
  volume={49 3},
  pages={227-37}
}
Two common viral diseases in psittacine birds including budgerigar fledgling disease (BFD), generally called avian polyomavirus (APV) infection, and psittacine beak and feather disease (PBFD) have similar clinical manifestations characterized by feather disorders. A duplex shuttle PCR was developed for detection of APV and PBFD virus (PBFDV). Two pairs of oligonucleotide primers were designed to amplify a 298-bp fragment of the t/T antigen region of APV genome and a 495-bp fragment of the… CONTINUE READING
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