Ductular network formation by rat biliary epithelial cells in the dynamical culture with collagen gel and dimethylsulfoxide stimulation.

@article{Hashimoto2008DuctularNF,
  title={Ductular network formation by rat biliary epithelial cells in the dynamical culture with collagen gel and dimethylsulfoxide stimulation.},
  author={Wataru Hashimoto and Ryo Sudo and Kazutomo Fukasawa and Mariko Ikeda and Toshihiro Mitaka and Kazuo Tanishita},
  journal={The American journal of pathology},
  year={2008},
  volume={173 2},
  pages={494-506}
}
Formation of bile ducts in culture is important for reconstructing hepatic organoids with bile drainage systems. However, morphogenic factors of biliary epithelial cells (BECs) have been poorly understood because of the lack of experimental models. Here, we demonstrated that rat BECs formed bile ductular networks in dynamic culture, when culture conditions were sequentially controlled. BEC morphogenesis was achieved through two-dimensional culture on collagen gel, collagen gel sandwich… CONTINUE READING