Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids

@article{Huang2015DuctalPC,
  title={Ductal pancreatic cancer modeling and drug screening using human pluripotent stem cell– and patient-derived tumor organoids},
  author={Ling Huang and Audrey Holtzinger and Ishaan C Jagan and Michael BeGora and Ines Lohse and Nicholas Ngai and Cristina Nostro and Rennian Wang and Lakshmi B. Muthuswamy and Howard C. Crawford and Cheryl H. Arrowsmith and Steve E Kalloger and Daniel John Renouf and Ashton A. Connor and Sean P. Cleary and David F. Schaeffer and Michael H Roehrl and Ming S Tsao and Steven Gallinger and G. Chi W.R. Keller and Senthil K Muthuswamy},
  journal={Nature Medicine},
  year={2015},
  volume={21},
  pages={1364-1371}
}
There are few in vitro models of exocrine pancreas development and primary human pancreatic adenocarcinoma (PDAC). We establish three-dimensional culture conditions to induce the differentiation of human pluripotent stem cells into exocrine progenitor organoids that form ductal and acinar structures in culture and in vivo. Expression of mutant KRAS or TP53 in progenitor organoids induces mutation-specific phenotypes in culture and in vivo. Expression of TP53R175H induces cytosolic SOX9… CONTINUE READING
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