Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells

@article{Salmon2002DualwavelengthFS,
  title={Dual-wavelength fluorescent speckle microscopy reveals coupling of microtubule and actin movements in migrating cells},
  author={Wendy C. Salmon and Michael C. Adams and Clare M. Waterman-Storer},
  journal={The Journal of Cell Biology},
  year={2002},
  volume={158},
  pages={31 - 37}
}
Interactions between microtubules (MTs) and filamentous actin (f-actin) are involved in directed cell locomotion, but are poorly understood. To test the hypothesis that MTs and f-actin associate with one another and affect each other's organization and dynamics, we performed time-lapse dual-wavelength spinning-disk confocal fluorescent speckle microscopy (FSM) of MTs and f-actin in migrating newt lung epithelial cells. F-actin exhibited four zones of dynamic behavior: rapid retrograde flow in… CONTINUE READING