Dual selective nitration in Arabidopsis: Almost exclusive nitration of PsbO and PsbP, and highly susceptible nitration of four non-PSII proteins, including peroxiredoxin II E.

@article{Takahashi2015DualSN,
  title={Dual selective nitration in Arabidopsis: Almost exclusive nitration of PsbO and PsbP, and highly susceptible nitration of four non-PSII proteins, including peroxiredoxin II E.},
  author={Misa Takahashi and Jun Shigeto and Atsushi Sakamoto and Shunsuke Izumi and Kozi Asada and Hiromichi Morikawa},
  journal={Electrophoresis},
  year={2015},
  volume={36 20},
  pages={2569-78}
}
Protein tyrosine nitration is a selective process, as revealed in studies of animals. However, evidence for selective protein nitration in plants is scarce. In this study, Arabidopsis plants were exposed to air with or without nitrogen dioxide at 40 ppm for 8 h in light. Proteins extracted from whole leaves or isolated chloroplasts were subjected to 2D PAGE followed by SYPRO Ruby staining and immunoblotting using an anti-3-nitrotyrosine antibody. We determined the relative intensity of a spot… CONTINUE READING

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