Dual screening for targeted gene replacement mutant in Magnaporthe oryzae with GUS as negative marker.

@article{Wang2009DualSF,
  title={Dual screening for targeted gene replacement mutant in Magnaporthe oryzae with GUS as negative marker.},
  author={Jiaoyu Wang and Zhen Zhang and Xinfa Du and Rongyao Chai and Xueqin Mao and Haiping Qiu and Yanli Wang and Guochang Sun},
  journal={Sheng wu gong cheng xue bao = Chinese journal of biotechnology},
  year={2009},
  volume={25 1},
  pages={129-38}
}
To improve the efficiency of targeted gene replacement (TGR), a dual screen (DS) system with gusA gene as negative selective marker (GUS-DS) was developed in Magnaporthe oryzae. First, we tested the endogenous beta-glucuronidase (GUS) activities of 78 fungal strains. All tested strains were GUS-, only with 3 exceptions. Whereas, after the gusA being… CONTINUE READING