Dual salt precipitation for the recovery of a recombinant protein from Escherichia coli.

@article{Balasundaram2011DualSP,
  title={Dual salt precipitation for the recovery of a recombinant protein from Escherichia coli.},
  author={Bangaru Balasundaram and Soam Sachdeva and Daniel G Bracewell},
  journal={Biotechnology progress},
  year={2011},
  volume={27 5},
  pages={
          1306-14
        }
}
When considering worldwide demand for biopharmaceuticals, it becomes necessary to consider alternative process strategies to improve the economics of manufacturing such molecules. To address this issue, the current study investigates precipitation to selectively isolate the product or remove contaminants and thus assist the initial purification of a intracellular protein. The hypothesis tested was that the combination of two or more precipitating agents will alter the solubility profile of the… CONTINUE READING