Dual pathways of glycerol assimilation in Klebsiella aerogenes NCIB 418

  title={Dual pathways of glycerol assimilation in Klebsiella aerogenes NCIB 418},
  author={Oense M. Neijssel and S. Hueting and K. J. Crabbendam and David Tempest},
  journal={Archives of Microbiology},
Klebsiella aerogenes NCIB 418 assimilates glycerol via alternative pathways: one involves a glycerol kinase with a high affinity for glycerol (apparent K m=1–2×10−6 M), and the second a glycerol dehydrogenase with a much lower affinity for its substrate (apparent K m=2–4×10−2 M). In variously-limited chemostat cultures, one or the other pathway predominated. Thus, aerobic carbonlimited organisms contained only the glycerol kinase pathway whereas aerobic sulphate-limited or ammonia-limited… CONTINUE READING


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