Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering.

@article{McMillan2018DualNG,
  title={Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering.},
  author={Alexandra McMillan and Minh K. Nguyen and Tomas Gonzalez-Fernandez and Peilin Ge and Xiaohua Yu and William L Murphy and Daniel J. Kelly and Eben Alsberg},
  journal={Biomaterials},
  year={2018},
  volume={161},
  pages={240-255}
}
High-density mesenchymal stem cell (MSC) aggregates can be guided to form bone-like tissue via endochondral ossification in vitro when culture media is supplemented with proteins, such as growth factors (GFs), to first guide the formation of a cartilage template, followed by culture with hypertrophic factors. Recent reports have recapitulated these results… CONTINUE READING