Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.

@article{Krel2007DualNA,
  title={Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.},
  author={Artur Krężel and Wolfgang Maret},
  journal={Journal of the American Chemical Society},
  year={2007},
  volume={129 35},
  pages={10911-21}
}
Each of the seven Zn(II) ions in the Zn(3)S(9) and Zn(4)S(11) clusters of human metallothionein is in a tetrathiolate coordination environment. Yet analysis of Zn(II) association with thionein, the apoprotein, and analysis of Zn(II) dissociation from metallothionein using the fluorescent chelating agents FluoZin-3 and RhodZin-3 reveal at least three classes of sites with affinities that differ by 4 orders of magnitude. Four Zn(II) ions are bound with an apparent average log K of 11.8, and with… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 52 extracted citations

Proteomic High Affinity Zn2+ Trafficking: Where Does Metallothionein Fit in?

International journal of molecular sciences • 2017
View 5 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…