Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.

  title={Dual nanomolar and picomolar Zn(II) binding properties of metallothionein.},
  author={Artur Krężel and Wolfgang Maret},
  journal={Journal of the American Chemical Society},
  volume={129 35},
Each of the seven Zn(II) ions in the Zn(3)S(9) and Zn(4)S(11) clusters of human metallothionein is in a tetrathiolate coordination environment. Yet analysis of Zn(II) association with thionein, the apoprotein, and analysis of Zn(II) dissociation from metallothionein using the fluorescent chelating agents FluoZin-3 and RhodZin-3 reveal at least three classes of sites with affinities that differ by 4 orders of magnitude. Four Zn(II) ions are bound with an apparent average log K of 11.8, and with… CONTINUE READING


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