Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.

  title={Dual multimodular class A penicillin-binding proteins in Mycobacterium leprae.},
  author={S Lepage and Philipe Dubois and Tapash K. Ghosh and Bernard Joris and Sebabrata Mahapatra and Mrityunjoy Kundu and J. P. Basu and Purbani Chakrabarti and Stewart T Cole and Martine Nguyen-Dist{\`e}che and J. M. Ghuysen},
  journal={Journal of bacteriology},
  volume={179 14},
The ponA gene of cosmid L222 of the Mycobacterium leprae genome library encodes a multimodular class A penicillin-binding protein (PBP), PBP1. The PBP, labelled with a polyhistidine sequence, has been produced in Escherichia coli, extracted from the membranes with 3-[(3-cholamidopropyl)-dimethylammonio]-1-propane-sulfonate (CHAPS) and purified by Ni2(+)-nitrilotriacetic acid-agarose chromatography. In contrast to the pon1-encoded class A PBP1, PBP1 undergoes denaturation at temperatures higher… CONTINUE READING

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Can penicillins and other β-lactam antibiotics be used to treat tuberculosis? Antimi-crob

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