Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae.

@article{McNabb2005DualLA,
  title={Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae.},
  author={David S. McNabb and Robin M. Reed and Robert A Marciniak},
  journal={Eukaryotic cell},
  year={2005},
  volume={4 9},
  pages={1539-49}
}
A new reporter system has been developed for quantifying gene expression in the yeast Saccharomyces cerevisiae. The system relies on two different reporter genes, Renilla and firefly luciferase, to evaluate regulated gene expression. The gene encoding Renilla luciferase is fused to a constitutive promoter (PGK1 or SPT15) and integrated into the yeast genome at the CAN1 locus as a control for normalizing the assay. The firefly luciferase gene is fused to the test promoter and integrated into the… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 36 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 39 references

Localization of a promoter in the putative internal ribosome entry site of the Saccharomyces cerevisiae TIF 4631 gene

  • H. Trachsel, M. Altmann
  • J . Bacteriol .
  • 2004

An in vivo dual - luciferase assay system for studying translational recoding in the yeast Saccharomyces cerevisiae

  • N. Wei, K. Keyomarsi
  • RNA
  • 2003

Similar Papers

Loading similar papers…