Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells.

@article{Rorsman1984DualEO,
  title={Dual effects of glucose on the cytosolic Ca2+ activity of mouse pancreatic beta-cells.},
  author={Patrik Rorsman and Hasse Abrahamsson and Erik Gylfe and B H Hellman},
  journal={FEBS letters},
  year={1984},
  volume={170 1},
  pages={196-200}
}
The cytosolic Ca2+ activity of mouse pancreatic beta-cells was studied with the intracellular fluorescent indicator quin2 . When the extracellular Ca2+ concentration was 1.20 mM, the basal cytosolic Ca2+ activity was 162 +/- 9 nM. Stimulation with 20 mM glucose increased this Ca2+ activity by 40%. In the presence of only 0.20 mM Ca2+ or after the addition of the voltage-dependent Ca2+ -channel blocker D-600, glucose had an opposite and more prompt effect in reducing cytosolic Ca2+ by about 15… CONTINUE READING