Dual-domain microchip-based process for volume reduction solid phase extraction of nucleic acids from dilute, large volume biological samples.

@article{Reedy2010DualdomainMP,
  title={Dual-domain microchip-based process for volume reduction solid phase extraction of nucleic acids from dilute, large volume biological samples.},
  author={Carmen R. Reedy and Kristin A Hagan and Briony C. Strachan and Joshua J Higginson and Joan M. Bienvenue and Susan A. Greenspoon and Jerome P Ferrance and James P. Landers},
  journal={Analytical chemistry},
  year={2010},
  volume={82 13},
  pages={5669-78}
}
A microfluidic device was developed to carry out integrated volume reduction and purification of nucleic acids from dilute, large volume biological samples commonly encountered in forensic genetic analysis. The dual-phase device seamlessly integrates two orthogonal solid-phase extraction (SPE) processes, a silica solid phase using chaotrope-driven binding and an ion exchange phase using totally aqueous chemistry (chitosan phase), providing the unique capability of removing polymerase chain… CONTINUE READING