Dual-color three-dimensional STED microscopy with a single high-repetition-rate laser.

Abstract

We describe a dual-color three-dimensional stimulated emission depletion (3D-STED) microscopy employing a single laser source with a repetition rate of 80 MHz. Multiple excitation pulses synchronized with a STED pulse were generated by a photonic crystal fiber, and the desired wavelengths were selected by an acousto-optic tunable filter with high spectral… (More)
DOI: 10.1364/OL.40.002653

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