Dual color localization microscopy of cellular nanostructures

  title={Dual color localization microscopy of cellular nanostructures},
  author={Manuel Gunkel and Fabian Erdel and Karsten Rippe and Paul Lemmer and Rainer Kaufmann and Christoph H{\"o}rmann and Roman Amberger and Christoph Cremer},
  journal={Biotechnology Journal},
The dual color localization microscopy (2CLM) presented here is based on the principles of spectral precision distance microscopy (SPDM) with conventional autofluorescent proteins under special physical conditions. This technique allows us to measure the spatial distribution of single fluorescently labeled molecules in entire cells with an effective optical resolution comparable to macromolecular dimensions. Here, we describe the application of the 2CLM approach to the simultaneous nanoimaging… 
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