Dual color localization microscopy of cellular nanostructures

@article{Gunkel2009DualCL,
  title={Dual color localization microscopy of cellular nanostructures},
  author={M. Gunkel and Fabian Erdel and K. Rippe and P. Lemmer and R. Kaufmann and Christoph H{\"o}rmann and R. Amberger and C. Cremer},
  journal={Biotechnology Journal},
  year={2009},
  volume={4}
}
The dual color localization microscopy (2CLM) presented here is based on the principles of spectral precision distance microscopy (SPDM) with conventional autofluorescent proteins under special physical conditions. This technique allows us to measure the spatial distribution of single fluorescently labeled molecules in entire cells with an effective optical resolution comparable to macromolecular dimensions. Here, we describe the application of the 2CLM approach to the simultaneous nanoimaging… Expand
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