Dual-axis tomography: an approach with alignment methods that preserve resolution.

  title={Dual-axis tomography: an approach with alignment methods that preserve resolution.},
  author={David N. Mastronarde},
  journal={Journal of structural biology},
  volume={120 3},
Tomographic reconstructions of biological specimens are now routinely being generated in our high voltage electron microscope by tilting the specimen around two orthogonal axes. Separate tomograms are computed from each tilt series. The two tomograms are aligned to each other with general 3-D linear transformations that can correct for distortions between the two tomograms, thus preserving the inherent resolution of the reconstruction throughout its volume. The 3-D Fourier transforms of the two… CONTINUE READING
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