Dual RNA-seq of pathogen and host

  title={Dual RNA-seq of pathogen and host},
  author={Alexander J Westermann and Stanislaw A. Gorski and J{\"o}rg Vogel},
  journal={Nature Reviews Microbiology},
A comprehensive understanding of host–pathogen interactions requires a knowledge of the associated gene expression changes in both the pathogen and the host. Traditional, probe-dependent approaches using microarrays or reverse transcription PCR typically require the pathogen and host cells to be physically separated before gene expression analysis. However, the development of the probe-independent RNA sequencing (RNA-seq) approach has begun to revolutionize transcriptomics. Here, we assess the… CONTINUE READING
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