Drusen proteome analysis: An approach to the etiology of age-related macular degeneration

@article{Crabb2002DrusenPA,
  title={Drusen proteome analysis: An approach to the etiology of age-related macular degeneration},
  author={J. Crabb and M. Miyagi and X. Gu and K. Shadrach and K. West and H. Sakaguchi and M. Kamei and A. Hasan and Lin Yan and M. Rayborn and R. G. Salomon and J. Hollyfield},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2002},
  volume={99},
  pages={14682 - 14687}
}
  • J. Crabb, M. Miyagi, +9 authors J. Hollyfield
  • Published 2002
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
Drusen are extracellular deposits that accumulate below the retinal pigment epithelium on Bruch's membrane and are risk factors for developing age-related macular degeneration (AMD). The progression of AMD might be slowed or halted if the formation of drusen could be modulated. To work toward a molecular understanding of drusen formation, we have developed a method for isolating microgram quantities of drusen and Bruch's membrane for proteome analysis. Liquid chromatography tandem MS analyses… Expand
Proteomic approaches to understanding age-related macular degeneration.
TLDR
Western blot analysis suggests that carboxyethyl pyrrole-protein adducts derived from docosahexaenoate-containing lipids are more abundant in AMD than in normal tissues, and may be causally involved in drusen formation and Bruch's membrane thickening. Expand
On the origin of proteins in human drusen: The meet, greet and stick hypothesis
TLDR
This work reviewed and supplemented the existing literature on the molecular composition of the retina/choroid complex and evaluated how drusen components may "meet, greet and stick" to each other and/or to structures like hydroxyapatite spherules to form macroscopic deposits in the sub-RPE-BL space. Expand
Quantitative proteomic analysis of aqueous humor from patients with drusen and reticular pseudodrusen in age-related macular degeneration
TLDR
Differential protein expression in the AH between patients with drusen and RPD was quantified using SWATH-MS, and detailed proteomic analyses of dry AMD patients might provide insights into the in vivo biology of drusan and R PD. Expand
Plasma Protein Pentosidine and Carboxymethyllysine, Biomarkers for Age-related Macular Degeneration*
TLDR
Overall this study supports the potential utility of plasma protein CML and pentosidine as biomarkers for assessing AMD risk and susceptibility, particularly in combination with CEP adducts and with concurrent analyses of fructosyl-lysine to detect confounding factors. Expand
Autophagy and Exosomes in the Aged Retinal Pigment Epithelium: Possible Relevance to Drusen Formation and Age-Related Macular Degeneration
TLDR
It is speculated that increased autophagy and the release of intracellular proteins via exosomes by the aged RPE may contribute to the formation of drusen. Expand
Quantitative Proteomics: Comparison of the Macular Bruch Membrane/Choroid Complex from Age-related Macular Degeneration and Normal Eyes*
TLDR
A quantitative proteomics analysis of the macular Bruch membrane/choroid complex was pursued, endorsing inflammatory processes in both early and advanced AMD pathology, implicate different pathways of progression to advanced dry and wet AMD, and provide a new database for hypothesis-driven and discovery-based studies of AMD. Expand
Proteomics of the retinal pigment epithelium reveals altered protein expression at progressive stages of age-related macular degeneration.
TLDR
These results provide the first direct evidence of AMD stage-specific changes in human RPE protein expression and provide a basis for functional investigation of AMD that may ultimately suggest new therapeutic strategies. Expand
Abundant Lipid and Protein Components of Drusen
TLDR
Manipulating genes encoding apolipoprotein pathways would be a fruitful approach to producing drusen with high EC content in laboratory animals, as at least 40% of druse content is comprised by lipids dominated by EC and PC. Expand
Mitochondrial proteomics of the retinal pigment epithelium at progressive stages of age-related macular degeneration.
TLDR
The results are consistent with the hypothesis that mitochondrial dysfunction is associated with AMD and further suggest specific pathophysiological mechanisms involving altered mitochondrial translation, import of nuclear-encoded proteins, and ATP synthase activity. Expand
Interaction of Complement Factor H and Fibulin3 in Age-Related Macular Degeneration
TLDR
The CFH 402H/Fib3 interaction could contribute to the development of pathological aggregates in soft drusen in some patients and as such might provide a target for therapeutic intervention in some forms of AMD. Expand
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Drusen associated with aging and age‐related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease
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TLDR
Drusen associated with aging and age‐related macular degeneration contain proteins common to extracellular deposits associated with atherosclerosis, elastosis, amyloidosis, and dense deposit disease. Expand
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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