Drosophila U6 promoter-driven short hairpin RNAs effectively induce RNA interference in Schneider 2 cells.

@article{Wakiyama2005DrosophilaUP,
  title={Drosophila U6 promoter-driven short hairpin RNAs effectively induce RNA interference in Schneider 2 cells.},
  author={Motoaki Wakiyama and Tomoko Matsumoto and Shigeyuki Yokoyama},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={331 4},
  pages={1163-70}
}
The effect of RNA interference (RNAi) is generally more potent in Drosophila Schneider 2 (S2) cells than in mammalian cells. In mammalian cells, PolIII promoter-based DNA vectors can be used to express small interfering RNA (siRNA) or short hairpin RNA (shRNA); however, this has not been demonstrated in cultured Drosophila cells. Here we show that shRNAs transcribed from the Drosophila U6 promoter can efficiently trigger gene silencing in S2 cells. By targeting firefly luciferase mRNA, we… CONTINUE READING

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