Drosophila Protein interaction Map (DPiM)

@article{Guruharsha2012DrosophilaPI,
  title={Drosophila Protein interaction Map (DPiM)},
  author={K. G. Guruharsha and Robert A. Obar and Julian Mintseris and K. Aishwarya and R. T. Krishnan and Krishnaswamy VijayRaghavan and Spyros Artavanis-Tsakonas},
  journal={Fly},
  year={2012},
  volume={6},
  pages={246 - 253}
}
Proteins perform essential cellular functions as part of protein complexes, often in conjunction with RNA, DNA, metabolites and other small molecules. The genome encodes thousands of proteins but not all of them are expressed in every cell type; and expressed proteins are not active at all times. Such diversity of protein expression and function accounts for the level of biological intricacy seen in nature. Defining protein-protein interactions in protein complexes, and establishing the when… 
Composition of the Survival Motor Neuron (SMN) Complex in Drosophila melanogaster
TLDR
Analysis of the composition of the SMN complex in Drosophila melanogaster reveals that epitope-tagged CG2941 interacts with endogenous SMN in Schneider2 cells, and RNAi-mediated knockdown ofCG2941 and its two close paralogs reveals that Gemin4 is essential for organismal viability.
Protein Kinase CK2: A Window into the Posttranslational Regulation of the E(spl)/HES Repressors from Invertebrates and Vertebrates
TLDR
It is suggested that E(spl)M8 is a paradigm for understanding the regulation of mammalian E( Spl) homologues by PTM, and this mode of regulation likely impacts a preponderance of E(Spl) members, underscoring its importance to Notch signaling.
Ush regulates hemocyte-specific gene expression, fatty acid metabolism and cell cycle progression and cooperates with dNuRD to orchestrate hematopoiesis
TLDR
It is revealed that Ush coordinates proliferation, metabolism and cell type-specific activities by isoform-specific cooperation with an epigenetic regulator, and specifically contributes to the repression of lineage-specific genes but does not impact the expression of cell cycle regulators or metabolic genes.
Cohesin occupancy and composition at enhancers and promoters are linked to DNA replication origin proximity in Drosophila
TLDR
It is demonstrated that Nipped-B and cohesin are differentially targeted to enhancers and promoters and suggested models for how SA and DNA replication help establish sister chromatid cohesion and facilitate enhancer-promoter communication are suggested.
Sequestration to lipid droplets promotes histone availability by preventing turnover of excess histones
TLDR
Using quantitative imaging, insight is provided into the mechanism for establishing histone stores during Drosophila oogenesis and light is shed on the function of LDs as protein-sequestration sites.
The Role of Systems Biologic Approach in Cell Signaling and Drug Development Responses—A Mini Review
TLDR
There is a need to form disease models outside of human subjects to check the effectiveness and response of the newer natural or synthetic chemicals.
Toward a Systems Understanding of Signaling Pathway Function.
Sequestration to lipid droplets promotes histone availability by preventing turnover of excess histones.
Because both dearth and overabundance of histones result in cellular defects, histone synthesis and demand are typically tightly coupled. In Drosophila embryos, histones H2B/H2A/H2Av accumulate on

References

SHOWING 1-10 OF 83 REFERENCES
Proteome survey reveals modularity of the yeast cell machinery
TLDR
This study reports the first genome-wide screen for complexes in an organism, budding yeast, using affinity purification and mass spectrometry and provides the largest collection of physically determined eukaryotic cellular machines so far and a platform for biological data integration and modelling.
A Protein Interaction Map of Drosophila melanogaster
TLDR
This map serves as a starting point for a systems biology modeling of multicellular organisms, including humans, and recapitulated known pathways, extended pathways, and uncovered previously unknown pathway components.
A Drosophila protein-interaction map centered on cell-cycle regulators
TLDR
The substantial number of new interactions discovered here supports the conclusion that previous interaction mapping studies were far from complete and that many more interactions remain to be found.
Global landscape of protein complexes in the yeast Saccharomyces cerevisiae
TLDR
T tandem affinity purification was used to process 4,562 different tagged proteins of the yeast Saccharomyces cerevisiae to identify protein–protein interactions, which will help future studies on individual proteins as well as functional genomics and systems biology.
Conserved patterns of protein interaction in multiple species.
TLDR
This comparison of the recently available protein-protein interaction networks of Caenorhabditis elegans, Drosophila melanogaster, and Saccharomyces cerevisiae revealed 71 network regions that were conserved across all three species and many exclusive to the metazoans.
A comprehensive two-hybrid analysis to explore the yeast protein interactome
TLDR
The comprehensive analysis using a system to examine two-hybrid interactions in all possible combinations between the budding yeast Saccharomyces cerevisiae is completed and would significantly expand and improve the protein interaction map for the exploration of genome functions that eventually leads to thorough understanding of the cell as a molecular system.
Towards a proteome-scale map of the human protein–protein interaction network
TLDR
An initial version of a proteome-scale map of human binary protein–protein interactions is described, which increases by ∼70% the set of available binary interactions within the tested space and reveals more than 300 new connections to over 100 disease-associated proteins.
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry
TLDR
Comparison of the HMS-PCI data set with interactions reported in the literature revealed an average threefold higher success rate in detection of known complexes compared with large-scale two-hybrid studies.
...
...