Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Abstract

Using three rounds of structure-guided directed evolution, the catalytic activity of the (S)-selective arylmalonate decarboxylase variant G74C/C188S could be increased up to 920-fold. The best variant had a 220-fold improved activity in the production of (S)-naproxen with excellent enantioselectivity (>99% ee). 
DOI: 10.1039/c1cc11953b

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@article{Miyauchi2011DramaticallyIC, title={Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.}, author={Yusuke Miyauchi and Robert Kourist and Daisuke Uemura and Kenji Miyamoto}, journal={Chemical communications}, year={2011}, volume={47 26}, pages={7503-5} }