History of Cell Culture
The end of twentieth century and early twenty‐first century brought the progress in 3‐D cell culture technology and created the possibility of the tissue engineering and the regenerative medicine development.
Protamine – A Review on an Oligonucleotide-Binding Peptide Applied in Nanopharmaceuticals including Vaccines
- Biology, Chemistry
Protamine-based RNA delivery systems in vaccines against infectious disease and their use in cancer treatment are reviewed and an update on the current state of latest developments with protamine as pharmaceutical excipient for vaccines is given.
Fibroblast-macrophage reciprocal interactions in health, fibrosis, and cancer.
- Medicine, BiologyImmunity
No Time to Age: Uncoupling Aging from Chronological Time
The plasticity of aging is considered as a process not fixed to the pace of chronological time but one that can speed up or slow down depending on the rate of intrinsic cellular clocks, which may eventually lead to therapeutic strategies to treat degenerative diseases by altering aging itself.
Senescence research from historical theory to future clinical application
- BiologyGeriatrics & gerontology international
Telomere lengths in human leukocytes correlate well with events of aging‐related lifestyle diseases, indicating the importance of cellular senescence in organismal aging.
Are Brain Organoids Equivalent to Philosophical Zombies?
It is crucial that ethical, legal and moral implications of organoid research are clear and that boundaries are set to separate scientific progress from human life preservation.
Die Geschichte der Zellkultur
- ArtDer Experimentator: Zellkultur
Die ersten Versuche, Gewebe bzw. Organe aus Spenderorganismen in vitrozu kultivieren, liegen schon sehr lange zuruck. Die Anfange der angewandten Gewebe- und Organkultur lassen sich etwa auf den…
In an effort to develop tissue culture techniques for long-term tissue cultivation , French surgeon and biologist
- Biology, Medicine
Carrel’s experiment convinced many biologists to accept immortality as an intrinsic property of all cells, not just the cell line through which genetic material is passed to offspring, called the germ line.
From organotypic culture to body‐on‐a‐chip: A neuroendocrine perspective
- BiologyJournal of neuroendocrinology
There will always be a need to consider the heterogeneous cellular compositions of organs in vivo, and modern microfluidic methodologies show promise for organ systems, ranging from the reproductive to the gastrointestinal to the brain.
Clonal evolution through genetic bottlenecks and telomere attrition: Potential threats to in vitro data reproducibility
- BiologyGenes, chromosomes & cancer
A list of factors to be avoided in order to reduce the risk of data misinterpretation due to clonal evolution, including unacknowledged in vitro selection pressures, prolonged culture per se, harsh population size reductions, experiments at early phases after establishment, and the employment of cell lines not sufficiently analyzed by high resolution genetic techniques are introduced.
SHOWING 1-10 OF 26 REFERENCES
Retention of differentiation potentialities during prolonged cultivation of myogenic cells.
- BiologyProceedings of the National Academy of Sciences of the United States of America
on the regulation of macromolecule synthesis during embryo-genesis that in several differentiating systems, proteins are formed on a stable form of RNA
Testing the commitment theory of cellular aging.
It is concluded that the limited growth of diploid fibroblasts is, in effect, an artifact of normal culturing procedures.
SV40-INDUCED TRANFORMATION OF HUMAN DIPLOID CELLS: CRISIS AND RECOVERY.
- BiologyJournal of cellular and comparative physiology
Crisis was characterized by progressive decline in vigor of culture proliferation, increasingly abnormal cell division, and limitation in propagability of cells eventually resulting in diminution in cell number.
Control of myogenesis in vitro by chick embryo extract.
- BiologyDevelopmental biology
PRESENT CONDITION OF A STRAIN OF CONNECTIVE TISSUE TWENTY-EIGHT MONTHS OLD
- BiologyThe Journal of experimental medicine
QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINES
- BiologyThe Journal of cell biology
Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending on the inoculation density, but nearly all cultures developed into established lines within 3 months of culture.
How the extract produces these effects [growth stimulation] is still wrapped in the mists of obscurity, which, although they frequently appear to be lifting, come down again as thick as ever
- How the extract produces these effects [growth stimulation] is still wrapped in the mists of obscurity, which, although they frequently appear to be lifting, come down again as thick as ever
Three lctures on the cultivation oftissues and tumours in vitro
80 Carrel, op. cit., note 26 above
- 80 Carrel, op. cit., note 26 above
cit., note 8 above
- cit., note 8 above