Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms’ Tumor 1 expression in K562 cells

  title={Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms’ Tumor 1 expression in K562 cells},
  author={Methee Rungrojsakul and Trinnakorn Katekunlaphan and Aroonchai Saiai and Chadarat Ampasavate and Siriporn Okonogi and Colleen Sweeney and Songyot Anuchapreeda},
  journal={BMC Complementary and Alternative Medicine},
BackgroundWilms’ tumor 1 (WT1) is a biological marker for predicting leukemia progression. In this study, mammea E/BB, an active compound from Saraphi (Mammea siamensis) seed extract was examined for its effect on down-regulatory mechanism of WT1 gene expression, WT1 protein and mRNA stability, and cell proliferation in K562 cell line.MethodsM. siamensis seeds were obtained from the region of Chiang Mai (North of Thailand). Mammea E/BB was extracted from seeds of M. siamensis. WT1 protein… Expand
Co-activation of WT1 and AP-1 proteins on WT1 gene promoter to induce WT1 gene expression in K562 cells.
AP-1 contributes to the WT1 autoregulation of WT1 gene expression in leukaemic K562 cells in a dose-dependent manner as shown by immunoblotting. Expand
Antileukemic Cell Proliferation of Active Compounds from Kaffir Lime (Citrus hystrix) Leaves
Antileukemic effects of the extracts and purified active compounds from the leaves and anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation are explored. Expand
Cytotoxicity and inhibition of leukemic cell proliferation by sesquiterpenes from rhizomes of Mah-Lueang (Curcuma cf. viridiflora Roxb.).
Four sesquiterpene compounds with antileukemic activities against the KG1a and Molt4 cell lines were identified in Mah-Lueang extracts. Expand
Acute toxicity and histopathological changes in livers of frog tadpoles (Hoplobatrachus rugulosus) exposed to bioinsecticides derived from Azadirachta indica A. Juss., Stemona curtisii Hook.F., and Mammea siamensis
From a higher value of LC50 of theNSAI than the SCMS together with the mild histopathological changes in the tadpoles exposed to the low concentrations, thus the NSAI is less toxic to H. rugulosus tadpole than theSCMS. Expand
Translational role of natural coumarins and their derivatives as anticancer agents.
This review hopes to stimulate and offer medicinal chemists and the reader in general an outlook on natural coumarins and their derivatives with potential for cancer therapy. Expand


Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line
Mammea siamensis seeds are identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression and to investigate its activity on Wilms' tumour 1 protein expression in K562 cells. Expand
Transcriptional regulation of the human Wilms' tumor gene (WT1). Cell type-specific enhancer and promiscuous promoter.
To develop an understanding of the role of WT1 in development and tumorigenesis, transcriptional regulatory elements that function in transient reporter gene constructs transfected into kidney and hematopoietic cell lines are identified and an essential promoter region by deletion analysis is identified. Expand
Antiproliferative and apoptotic effects of compounds from the flower of Mammea siamensis (Miq.) T. Anders. on human cancer cell lines.
Results suggest that compound 3, 4, and 5 exert antiproliferative actions through apoptotic cell death in leukemia cells and these compounds may have the potential to be developed into new anti-cancer drug candidates. Expand
Mammea E/BB, an isoprenylated dihydroxycoumarin protonophore that potently uncouples mitochondrial electron transport, disrupts hypoxic signaling in tumor cells.
Mechanistic studies revealed that mammea-type coumarin 1 appears to exert an assemblage of cellular effects by functioning as an anionic protonophore that potently uncouples mitochondrial electron transport and disrupts mitochondrial signaling in human tumor cell lines. Expand
Effect of pure curcumin, demethoxycurcumin, and bisdemethoxycurcumin on WT1 gene expression in leukemic cell lines
PureCurcumin, an excellent curcuminoid derivative, decreased WT1 gene expression in both transcriptional and translational levels and is one of a potential chemotherapeutic agent used for treatment of human leukemia. Expand
Inhibitory effect of curcumin onWT1 gene expression in patient leukemic cells
Curcumin treatment may provide a lead for clinical treatment in leukemic patients in the future as curcumin decreased WT1 mRNA in patientLeukemic cells. Expand
The role of the Wilms tumour gene (WT1) in normal and malignant haematopoiesis
The ubiquity of WT1 in haematological malignancies has led to efforts to exploit it as a marker for minimal residual disease and as a prognostic factor, with conflicting results. Expand
Natural and semisynthetic mammea-type isoprenylated dihydroxycoumarins uncouple cellular respiration.
Mechanistic studies indicate that mammea-type coumarins act as anionic protonophores that potently uncouple mitochondrial electron transport and disrupt hypoxic signaling. Expand
Inhibitory mechanism of pure curcumin on Wilms’ tumor 1 (WT1) gene expression through the PKCα signaling pathway in leukemic K562 cells
Ch Chromatin immunoprecipitation and reporter gene assays indicate that pure curcumin treatment attenuates WT1 auto‐regulation through inhibition of PKCα signaling in K562 cells. Expand
[WT1 gene expression in leukemia patients and its correlation with prognosis and multidrug resistance].
  • X. Guo, S. Xu, Z. Dong
  • Biology, Medicine
  • Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
  • 1999
The expression of WT1 can predict the treatment outcome and the prognosis for leukemia patients, and the coexpression ofWT1 and bcl-2 predicts poor prognosis of AML patients. Expand