Down-regulation of opiate receptors in serum-free cultures of aggregating fetal brain cells.

@article{Lenoir1983DownregulationOO,
  title={Down-regulation of opiate receptors in serum-free cultures of aggregating fetal brain cells.},
  author={Dominique Lenoir and Jacob Barg and Rabi Simantov},
  journal={Life sciences},
  year={1983},
  volume={33 Suppl 1},
  pages={
          337-40
        }
}
Brain cells from rat embryos that were cultured in serum-free medium form aggregates which possess stereospecific binding of radioactive opiates. The specificity of these opiate receptors and their regulation by opiate alkaloids and enkephalin was studied. D-ala2, D-leu5-enkephalin (DADL) induced down-regulation of opiate receptors in aggregates prepared from the forebrain but had no effect on hindbrain aggregates, whereas the potent alkaloid etorphine induced down-regulation of the receptors… CONTINUE READING