Down-regulation of myosin light chain kinase expression in vascular smooth muscle cells accelerates cell proliferation: requirement of its actin-binding domain for reversion to normal rates.

@article{Wang2012DownregulationOM,
  title={Down-regulation of myosin light chain kinase expression in vascular smooth muscle cells accelerates cell proliferation: requirement of its actin-binding domain for reversion to normal rates.},
  author={Hong Hui Wang and Akio Nakamura and Shinji Yoshiyama and Ryoki Ishikawa and Na Cai and Li-hong Ye and Hiromi Takano-Ohmuro and Kazuhiro Kohama},
  journal={Journal of pharmacological sciences},
  year={2012},
  volume={119 1},
  pages={91-6}
}
Myosin light-chain kinase (MLCK) is a multi-domain protein with kinase and actin-binding domains, among others. Deficiency of MLCK expression in GBaSM-4 vascular smooth muscle cells enhanced cell proliferation rate and shortened cell doubling time. Transient transfection of the MLCK-deficient cells with cDNA constructs of either wild-type MLCK or its mutant lacking the kinase activity reverted the cell proliferation rate to that of wild-type cells, whereas that of MLCK lacking the actin-binding… CONTINUE READING