Down-regulation of cell surface insulin receptors by sarco(endo)plasmic reticulum Ca2+-ATPase inhibitor in adrenal chromaffin cells.

@article{Shiraishi2001DownregulationOC,
  title={Down-regulation of cell surface insulin receptors by sarco(endo)plasmic reticulum Ca2+-ATPase inhibitor in adrenal chromaffin cells.},
  author={Seiji Shiraishi and Ryuichi Yamamoto and Toshihiko Yanagita and Hiroki Yokoo and Hideyuki Kobayashi and Yasuhito Uezono and Akihiko Wada},
  journal={Brain research},
  year={2001},
  volume={898 1},
  pages={152-7}
}
Long-term (> or =12 h) treatment of cultured bovine adrenal chromaffin cells with thapsigargin (TG), an inhibitor of sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA), caused a time (t(1/2)=16.3 h)- and concentration (IC50=37.8 nM)-dependent decrease of cell surface 125I-insulin binding by 35%, but did not change the Kd value. TG caused a sustained increase of cytoplasmic concentration of Ca2+ ([Ca2+]c) in a biphasic manner, and the effect of TG on 125I-insulin binding was abolished by BAPTA-AM… CONTINUE READING