Down-regulation of Smad4 enhances proliferation and invasion of colorectal carcinoma HCT116 cells and up-regulates Id2.

@article{Shi2012DownregulationOS,
  title={Down-regulation of Smad4 enhances proliferation and invasion of colorectal carcinoma HCT116 cells and up-regulates Id2.},
  author={Qiang Shi and Yun-shi Zhong and Li-qing Yao and Quanlin Li and Zhong Ren and Xiang-ping Liu and Fa-Mao Shi},
  journal={Molecular medicine reports},
  year={2012},
  volume={5 1},
  pages={89-95}
}
The aim of this study was to determine whether the suppression of Smad4 by short hairpin RNA (shRNA) regulates the proliferation and invasion of colorectal carcinoma HCT116 cells and Id2 expression. The Smad4‑shRNA expression vectors were constructed and stably transfected to HCT116 cells. The expression of mRNA and protein of Smad4 and Id2 was detected using reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting… CONTINUE READING

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Therefore , Smad4 may act as a tumor - suppressor gene in colorectal carcinoma .
Therefore , Smad4 may act as a tumor - suppressor gene in colorectal carcinoma .
The expression of mRNA and protein of Smad4 and Id2 was detected using reverse transcription - polymerase chain reaction ( RT - PCR ) and Western blotting .
The expression of mRNA and protein of Smad4 and Id2 was detected using reverse transcription - polymerase chain reaction ( RT - PCR ) and Western blotting .
The expression of mRNA and protein of Smad4 and Id2 was detected using reverse transcription - polymerase chain reaction ( RT - PCR ) and Western blotting .
The expression of mRNA and protein of Smad4 and Id2 was detected using reverse transcription - polymerase chain reaction ( RT - PCR ) and Western blotting .
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