Down-regulation of PARP-1, but not of Ku80 or DNA-PKcs', results in higher gene targeting efficiency.

@article{DominguezBendala2006DownregulationOP,
  title={Down-regulation of PARP-1, but not of Ku80 or DNA-PKcs', results in higher gene targeting efficiency.},
  author={Juan Dominguez-Bendala and Mitsuko Masutani and Jim McWhir},
  journal={Cell biology international},
  year={2006},
  volume={30 4},
  pages={389-93}
}
The viability of non-homologous end-joining (NHEJ)-defective mice suggests that homologous recombination (HR) might take over its role in DNA repair. To test this hypothesis, we examined gene targeting frequencies (TF) in DNA-PK(cs), Ku80 and poly(ADP-ribose) polymerase (PARP-1) nullizygous cells. We observed a 3-fold TF increase in PARP-1 knockout… CONTINUE READING