Down-regulation and CpG island hypermethylation of CRYAA in age-related nuclear cataract.

@article{Zhou2012DownregulationAC,
  title={Down-regulation and CpG island hypermethylation of CRYAA in age-related nuclear cataract.},
  author={Peng Zhou and Yi Luo and Xin Liu and Lei Fan and Yi Lu},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2012},
  volume={26 12},
  pages={4897-902}
}
Age-related cataract (ARC) is the leading cause of blindness among older adults, and the chaperone-like activity of αA-crystallin (CRYAA) is considered to be critical for the maintenance of eye lens transparency. To assess the potential contribution of epigenetic regulation of CRYAA genes relevant to ARC pathogenesis, we evaluated DNA methylation, a tissue-specific genetic modulation that affects gene expression. Reverse-transcription PCR and Western blot were used to analyze the expression of… CONTINUE READING