Down‐regulation of the strawberry Bet v 1‐homologous allergen in concert with the flavonoid biosynthesis pathway in colorless strawberry mutant

@article{Hjern2006DownregulationOT,
  title={Down‐regulation of the strawberry Bet v 1‐homologous allergen in concert with the flavonoid biosynthesis pathway in colorless strawberry mutant},
  author={Karin Hjern{\o} and Rikard Alm and Bj{\"o}rn Canb{\"a}ck and Rune Matthiesen and K. Trajkovski and Lars Bj{\"o}rk and Peter Roepstorff and Cecilia Emanuelsson},
  journal={PROTEOMICS},
  year={2006},
  volume={6}
}
Proteomic screening of strawberry (Fragaria ananassa) yielded a 58% success rate in protein identification in spite of the fact that no genomic sequence is available for this species. This was achieved by a combination of MALDI‐MS/MS de novo sequencing of double‐derivatized peptides and indel‐tolerant searching against local protein databases built on both EST and full‐length nucleotide sequences. The amino acid sequence of a strawberry allergen, homologous to the well‐known major birch pollen… 
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The strawberry fruit Fra a allergen functions in flavonoid biosynthesis.
TLDR
The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.
Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in strawberry (Fragaria ananassa) shows the presence of an intron and little variability in amino acid sequence.
Genomic identification of putative allergen genes in woodland strawberry ( Fragaria vesca ) and mandarin orange ( Citrus clementina )
TLDR
Comparative genomic analysis of the allergen genes and encoded proteins in woodland strawberry and mandarin orange provides a basis for future research on developing low-allergenic cultivars without compromising the natural defense of plants.
The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response
TLDR
Gene ontology analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological process, and redox state was not apparently altered since no changes were found in ascorbic acid and glutathione reduced/oxidized ratio, but GSH content was reduced in the silenced fruits.
Bet v 1 potential allergens are involved in anthracnose resistance of strawberry varieties
TLDR
Investigation of whether Bet v 1 potential allergens participate in the resistance of berry crops against fungal pathogen finds their correspondence with anthracnose resistance that might be instructive to future breeding strategies.
Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria × ananassa
TLDR
Based on the localizations, accumulation levels and reactivity to human IgE, it was determined that Fra a 1.01 was the most important allergen, followed by Fra a 2.02, and then otherFra a 1 proteins.
The Strawberry Pathogenesis-related 10 (PR-10) Fra a Proteins Control Flavonoid Biosynthesis by Binding to Metabolic Intermediates*
TLDR
These findings provide mechanistic insight on the function of Fra a proteins and suggest that PR-10 proteins, which are widespread in plants, may play a role in the control of secondary metabolic pathways by binding to metabolic intermediates.
Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP: Interaction of Fra a 1 proteins and FaAP
TLDR
The results imply that PR‐10 proteins might be integrated into a protein‐interaction network and FaAP binding appears to be essential for the physiological function of the Fra a 1 proteins.
Solution structure of the strawberry allergen Fra a 1
TLDR
The three-dimensional structure of Fra a 1E contains the glycine-rich loop that is highly conserved throughout the protein family, and the volume of the hydrophobic pocket and the size of its entrance are much larger than expected.
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