Down‐regulation of the strawberry Bet v 1‐homologous allergen in concert with the flavonoid biosynthesis pathway in colorless strawberry mutant

@article{Hjern2006DownregulationOT,
  title={Down‐regulation of the strawberry Bet v 1‐homologous allergen in concert with the flavonoid biosynthesis pathway in colorless strawberry mutant},
  author={Karin Hjern{\o} and Rikard Alm and Bj{\"o}rn Canb{\"a}ck and Rune Matthiesen and K. Trajkovski and Lars Bj{\"o}rk and Peter Roepstorff and Cecilia Emanuelsson},
  journal={PROTEOMICS},
  year={2006},
  volume={6}
}
Proteomic screening of strawberry (Fragaria ananassa) yielded a 58% success rate in protein identification in spite of the fact that no genomic sequence is available for this species. This was achieved by a combination of MALDI‐MS/MS de novo sequencing of double‐derivatized peptides and indel‐tolerant searching against local protein databases built on both EST and full‐length nucleotide sequences. The amino acid sequence of a strawberry allergen, homologous to the well‐known major birch pollen… 
The strawberry fruit Fra a allergen functions in flavonoid biosynthesis.
TLDR
The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.
Genomic identification of putative allergen genes in woodland strawberry ( Fragaria vesca ) and mandarin orange ( Citrus clementina )
TLDR
Comparative genomic analysis of the allergen genes and encoded proteins in woodland strawberry and mandarin orange provides a basis for future research on developing low-allergenic cultivars without compromising the natural defense of plants.
Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response
TLDR
Gene ontology analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological process, and redox state was not apparently altered since no changes were found in ascorbic acid and glutathione reduced/oxidized ratio, but GSH content was reduced in the silenced fruits.
Bet v 1 potential allergens are involved in anthracnose resistance of strawberry varieties
TLDR
Investigation of whether Bet v 1 potential allergens participate in the resistance of berry crops against fungal pathogen finds their correspondence with anthracnose resistance that might be instructive to future breeding strategies.
Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria × ananassa
TLDR
Based on the localizations, accumulation levels and reactivity to human IgE, it was determined that Fra a 1.01 was the most important allergen, followed by Fra a 2.02, and then otherFra a 1 proteins.
The Strawberry Pathogenesis-related 10 (PR-10) Fra a Proteins Control Flavonoid Biosynthesis by Binding to Metabolic Intermediates*
TLDR
These findings provide mechanistic insight on the function of Fra a proteins and suggest that PR-10 proteins, which are widespread in plants, may play a role in the control of secondary metabolic pathways by binding to metabolic intermediates.
Physical interaction between the strawberry allergen Fra a 1 and an associated partner FaAP: Interaction of Fra a 1 proteins and FaAP
TLDR
The results imply that PR‐10 proteins might be integrated into a protein‐interaction network and FaAP binding appears to be essential for the physiological function of the Fra a 1 proteins.
Solution structure of the strawberry allergen Fra a 1
TLDR
The three-dimensional structure of Fra a 1E contains the glycine-rich loop that is highly conserved throughout the protein family, and the volume of the hydrophobic pocket and the size of its entrance are much larger than expected.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 43 REFERENCES
Novel isoforms of Pru av 1 with diverging immunoglobulin E binding properties identified by a synergistic combination of molecular biology and proteomics
TLDR
The data demonstrate that a synergistic combination of molecular biology and proteomics represents a powerful tool for reliable and comprehensive identification of allergen isoforms and variants and may be relevant for the diagnosis or therapy of cherry allergy.
Molecular and biochemical classification of plant-derived food allergens.
TLDR
Allergens other than PR homologs can be allotted to other well-known protein families such as inhibitors of alpha-amylases and trypsin from cereal seeds, profilins from fruits and vegetables, seed storage proteins from nuts and mustard seeds, and proteases from fruits.
The Major Birch Allergen, Bet v 1, Shows Affinity for a Broad Spectrum of Physiological Ligands*
TLDR
It is indicated that ligand binding is important for the biological function of Bet v 1, a 17-kDa protein abundantly present in the pollen of the White birch tree and the primary cause of birch pollen allergy in humans.
Pollen-related food allergy: cloning and immunological analysis of isoforms and mutants of Mal d 1, the major apple allergen, and Bet v 1, the major birch pollen allergen
TLDR
It is concluded that divergent allergenicity of apple strains mainly depends on differnet expression levels of the majorAllergen, and that mutants with reduced IgE-reactivity but maintained T-cell reactivity may represent new candidates for a safer specific immunotherapy with reduced side-effects.
Characterization of the 18-kDa apple allergen by two-dimensional immunoblotting and microsequencing.
TLDR
The results support the view that both proteins express common as well as non-related IgE-reactive epitopes on Bet v I related epitopes.
Molecular and Biochemical Characterization of an Enzyme Responsible for the Formation of Hypericin in St. John's Wort (Hypericum perforatum L.)*
TLDR
A direct and complex conversion of emodin to HyH that is solely catalyzed by Hyp-1, a Bet.v.1 class allergen from H. perforatum is demonstrated.
Expression of Genes Involved in Anthocyanin Biosynthesis in Relation to Anthocyanin, Proanthocyanidin, and Flavonol Levels during Bilberry Fruit Development1
TLDR
A correlation between anthocyanin accumulation and expression of the flavonoid pathway genes during the ripening of berries is demonstrated and models for the connection between flavonol and anthOCyanin syntheses in fruit tissues are presented.
An intragenic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers pale-colored flowers and seeds with fine spots in Ipomoea tricolor.
TLDR
The flower and seed variegation studied here are likely to be caused by somatic homologous recombination between an intragenic tandem duplication in the gene encoding a bHLH transcriptional activator for anthocyanin biosynthesis, whereas various flower variegations are reported to be causing by excision of DNA transposons inserted into pigmentation genes.
ANTHOCYANIN1 of Petunia Controls Pigment Synthesis, Vacuolar pH, and Seed Coat Development by Genetically Distinct Mechanisms Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.003772.
TLDR
It is shown that AN1 controls additional aspects of cell differentiation: the acidification of vacuoles in petal cells, and the size and morphology of cells in the seed coat epidermis.
Current Understanding of Cross‐Reactivity of Food Allergens and Pollen
TLDR
Approaches to introduce such proteins as a measure to protect plants against diseases should be performed with caution as they may increase the allergenicity of these crops.
...
1
2
3
4
5
...