Doublet discrimination in DNA cell-cycle analysis.

  title={Doublet discrimination in DNA cell-cycle analysis.},
  author={Robert P. Wersto and Francis J. Chrest and James F. Leary and Christopher Morris and Maryalice Stetler-Stevenson and Edward W. Gabrielson},
  volume={46 5},
Differences in doublet analysis have the potential to alter DNA cell-cycle measurements. The techniques for doublet determination are often used interchangeably without regard for the complexity in cell shapes and sizes of biological specimens. G(0/1) doublets were identified and quantitated using fluorescence height versus area and fluorescence width versus area pulse measurements, by enumerating the proportion of G(2) + M cells that lack cyclin B1 immunoreactivity, and modeled in the DNA… CONTINUE READING