Double whole mount in situ hybridization of early chick embryos.

  title={Double whole mount in situ hybridization of early chick embryos.},
  author={Delphine Psychoyos and Richard H. Finnell},
  journal={Journal of visualized experiments : JoVE},
The chick embryo is a valuable tool in the study of early embryonic development. Its transparency, accessibility and ease of manipulation, make it an ideal tool for studying gene expression in brain, neural tube, somite and heart primordia formation. This video demonstrates the different steps in 2-color whole mount in situ hybridization; First, the embryo is dissected from the egg and fixed in paraformaldehyde. Second, the embryo is processed for prehybridization. The embryo is then hybridized… CONTINUE READING

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