Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells.

@article{Wang2008DoublestrandedBC,
  title={Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells.},
  author={Zai Wang and Zhong-Jun Zhou and De-Pei Liu and Junji Huang},
  journal={Oligonucleotides},
  year={2008},
  volume={18 1},
  pages={21-32}
}
Single-stranded oligonucleotide (SSO)-mediated gene modification is a newly developed tool for site-specific gene repair in mammalian cells; however, the corrected cells always show G2/M arrest and cannot divide to form colonies. This phenomenon and the unclear mechanism seriously challenge the future application of this technique. In this study, we developed an efficient SSO-mediated DNA repair system based on double-stranded break (DSB) induction. We generated a mutant EGFP gene with… CONTINUE READING
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