Double-stranded RNA mediates homology-dependent gene silencing of gamma-tubulin in the human parasite Entamoeba histolytica.

@article{Vayssi2004DoublestrandedRM,
  title={Double-stranded RNA mediates homology-dependent gene silencing of gamma-tubulin in the human parasite Entamoeba histolytica.},
  author={Laurence Vayssi{\'e} and Miguel R Vargas and Christian Weber and Nancy Guillen},
  journal={Molecular and biochemical parasitology},
  year={2004},
  volume={138 1},
  pages={21-8}
}
Approaches that eliminate mRNA are a powerful tool for reverse genetics applications in eukaryotic microbes for which gene replacement techniques have not yet been developed. Here, for the first time, we demonstrate that RNA duplexes efficiently inhibit gene expression when introduced into the human parasite Entamoeba histolytica. Chemically synthesized… CONTINUE READING