Double-strand breaks on artificial chromosomes in yeast

@article{Ross2000DoublestrandBO,
  title={Double-strand breaks on artificial chromosomes in yeast},
  author={Lyle O. Ross and Drora Zenvirth and Ana Rita Jardim and Dean Dawson},
  journal={Chromosoma},
  year={2000},
  volume={109},
  pages={226-234}
}
Yeast artificial chromosomes composed primarily of bacteriophage λ DNA exhibit very low levels of meiotic crossing over compared with similarly sized intervals of natural yeast DNA. When these recombinationally quiet chromosomes were augmented with a 12.5 kb insert of sequences from yeast chromosome VIII, genetic studies demonstrated that the artificial chromosomes had acquired recombination properties characteristic of this region of chromosome VIII. On authentic yeast chromosomes, most… CONTINUE READING

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