Double staining of immunoblot using enzyme histochemistry and India ink.

@article{Ono1990DoubleSO,
  title={Double staining of immunoblot using enzyme histochemistry and India ink.},
  author={Takashi Ono and Rocky S Tuan},
  journal={Analytical biochemistry},
  year={1990},
  volume={187 2},
  pages={324-7}
}
Immunoblotting is a commonly used technique for the immunodetection of specific proteins which have been fractionated by polyacrylamide gel electrophoresis. We describe here a simple procedure for the double staining of immunoblots, first to detect the immunoreactive component(s) by histochemistry using enzyme-conjugated secondary antibodies, and second to… CONTINUE READING