Double-origin vectors for isolating bidirectional deletions useful in DNA sequence analysis.

Abstract

Cosmids containing two compatible origins of replication (oris) have been constructed for isolating bidirectional deletions in cloned DNA. The inserted fragment and the cos site are cut, respectively, with a restriction enzyme and lambda terminase to produce two linear fragments that are circularized to produce two autonomously replicating plasmids. Each… (More)

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