Double minutes arise from circular extrachromosomal DNA intermediates which integrate into chromosomal sites in human HL-60 leukemia cells.

@article{Hoff1990DoubleMA,
  title={Double minutes arise from circular extrachromosomal DNA intermediates which integrate into chromosomal sites in human HL-60 leukemia cells.},
  author={D. D. Von Hoff and Barbara Forseth and Carl Clare and K L Hansen and Donald R VanDevanter},
  journal={The Journal of clinical investigation},
  year={1990},
  volume={85 6},
  pages={1887-95}
}
Amplification of oncogenes has been found to be an important prognostic factor in behavior of patients' malignancies. In this study we have used new gel electrophoresis techniques to follow the location of amplified c-myc oncogene sequences in HL-60 promyelocytic leukemia cells. In passages 46-62 of the cells, the cells contain amplified c-myc sequences on submicroscopic circular extrachromosomal DNA (episomes). With increased passages in culture (passages 63-72) the cells lose the episome c… CONTINUE READING