Double labeling with iodo- and bromodeoxyuridine for cell kinetics studies.

@article{Shibui1989DoubleLW,
  title={Double labeling with iodo- and bromodeoxyuridine for cell kinetics studies.},
  author={Soichiro Shibui and Takao Hoshino and Marty Vanderlaan and Joe W. Gray},
  journal={The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society},
  year={1989},
  volume={37 7},
  pages={1007-11}
}
The rate of progression through the cell cycle was determined in five human glioma cell lines by a new sequential immunohistochemical staining technique. The cells were labeled first with iododeoxyuridine (IdUrd) for 1-3 hr and then with bromodeoxyuridine (BrdUrd) for 30 min. Labeled cells were identified with Br-3, a monoclonal antibody that recognizes only BrdUrd, and with IU-4, an antibody that recognizes both IdUrd and BrdUrd. Each slide was stained sequentially, first with the… CONTINUE READING

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