Double knockout pigs deficient in N-glycolylneuraminic acid and galactose α-1,3-galactose reduce the humoral barrier to xenotransplantation.

@article{Lutz2013DoubleKP,
  title={Double knockout pigs deficient in N-glycolylneuraminic acid and galactose α-1,3-galactose reduce the humoral barrier to xenotransplantation.},
  author={Andrew J Lutz and Ping Li and Jose L. Estrada and Richard A. Sidner and Ray K. Chihara and Susan M Downey and Christopher Burlak and Zheng-yu Wang and Luz McNaughton Reyes and Bess Ivary and Fuqin Yin and Ross L Blankenship and Leela L Paris and Alfred Joseph Tector},
  journal={Xenotransplantation},
  year={2013},
  volume={20 1},
  pages={27-35}
}
BACKGROUND Clinical xenotransplantation is not possible because humans possess antibodies that recognize antigens on the surface of pig cells. Galα-1,3-Gal (Gal) and N-glycolylneuraminic acid (Neu5Gc) are two known xenoantigens. METHODS We report the homozygous disruption of the α1, 3-galactosyltransferase (GGTA1) and the cytidine monophosphate-N-acetylneuraminic acid hydroxylase (CMAH) genes in liver-derived female pig cells using zinc-finger nucleases (ZFNs). Somatic cell nuclear transfer… CONTINUE READING
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