Double in situ hybridization techniques in zebrafish.

@article{Jowett2001DoubleIS,
  title={Double in situ hybridization techniques in zebrafish.},
  author={T. Jowett},
  journal={Methods},
  year={2001},
  volume={23 4},
  pages={345-58}
}
Over recent years many genes expressed in zebrafish embryos have been cloned and a first step in their analysis is the determination of the spatial and temporal expression patterns of their transcripts by in situ hybridization. It is often necessary to relate the expression pattern to that of other genes expressed at the same period of development. Transcripts from different genes may be expressed in complementary or overlapping domains. The pattern of expression of different genes can be… CONTINUE READING