Double-immunodiffusion assay for detecting specific antibodies.

  title={Double-immunodiffusion assay for detecting specific antibodies.},
  author={Peter Hornbeck},
  journal={Current protocols in immunology},
  volume={Chapter 2},
  pages={Unit 2.3}
Double immunodiffusion owes its success to the unique nature of antibody-antigen interactions. When polyvalent antibodies with moderate-to-high intrinsic affinities are mixed with antigen at the right ratio (called the zone of equivalence) lattices of antibody-antigen complexes form and precipitate out of solution. When, as described in this unit, gradients of antigen and antibody are established by diffusion from adjacent wells in a bed of agar, a line of practically insoluble precipitation… CONTINUE READING

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